Cyphobasidium puncteliicola Diederich & W.R.Buck

• Region

  North America

• Country

  United States of America

• State/Province

  Missouri

• County/Municipio

  Dade Co.

• Locality

  Bona Glade Natural Area, along MO 215 just E of bridge over Maze Creek Arm of Stockton Lake

• Township, Range, Section

  T33N, R25W, sec. 32 SE 1/4

• Coordinates

  37.5453, -93.6917

• Location Notes

  [Map associated with specimen]

• Distribution

  Map all specimens of this taxon

The New York Botanical Garden
Lichens of Missouri, U.S.A.
Tremella
on Punctelia graminicola (de Lesd.) Egan
Dade County: Bona Glade Natural Area, along MO
215 just E of bridge over Maze Creek Arm of
Stockton Lake, 37¦32Æ43"N, 93¦41Æ30"W, T.33N.,
R.25W., sect. 32 SEV4; channel sandstone glade with
shaded sandstone bluffs.
16 April 2005
Richard C. Harris 50901
Collected with support from the National Science Foundation for "Lichens of the Ozarksö
NEW YORK BOTANICAL GARDEN
078299
HOLOTYPE OF:
Cyphobasidium puncteliicola Diederich & Millanes
See. Diederich, P., A.M. Millanes, A. Flakus, P. Rodriguez-Flakus, J. Etayo
and M. Wedin. 2022. Class Cystobasidiomycetes, order Cyphobasidiales.
Flora of Lichenicolous Fungi li 305-324. [on p. 318]
Det. J.C. Lendemer, 2022
septum; epibasidia-like structures more or less perpendicular
to the basidium or directed upwards, cylindrical, 3-4 pm thick,
13.5-22.5 pm long, possibly representing secondary meiospo-
rangia. Basidiospores not observed. Asexual stage unknown.
Notes. This species is distinguished from all known lichen-
icolous Cyphobasidium species by the very thick and long
epibasidia-like structures that might function as secondary
meiosporangia. No basidiospores have been observed.
Ecology and host. On the thallus of Lecania rutegelii on
thin branches of Quercus.
Distribution. Europe (BelgiumX known only from the type locality.
Cyphobasidium puncteliicola Diederich & Millanes, sp. nov.
Diagnosis: Characterized by the basidiomata inducing galls with
a constricted base, first 0.3-0.4 mm diam., concolorous to the
host thallus, soon partly dark brown, eventually lobifbrm with
a concave to fbveolate upper surface and up to 1 mm diam., on
the thallus of Punctelia graminicola, ellipsoid probasidia, 9-17
x 5.5-11 pm, cylindrical, 2-3-transseptate meiosporangia, 25-
40 x 4-5 pm, short epibasidia, 1-2 pm thick and 4-5 pm long,
and ellipsoid to fusiform basidiospores, 5-9 x 4-6.5 pm.
Etymology: From Punctelia, the host lichen, and incola, dweller.
T)>pe: USA, Missouri, Dade Ch., Bona Glade Natural Area, along
MO 215 just E of bridge over Maze Creek Arm of Stockton
Lake, 37¦32'43öN, 93¦41 Æ30öW, channel sandstone glade with
Cyphobasidium puncteliicola, USA, Missouri, holotype. Basidiomatal galls on the thallus of Punctelia graminicola. Scale bar: 200 pm.
Cyphobasidium puncteliicola, USA, Missouri, holotype. A, Subbasidial hyphae with probasidia. B, Probasidia with young emetging meiosno-
rangia. C, Basidiospores. D, Meiosporangia. E, Meiosporangium with lateral epibasidium and basidiospore. C, in Congo red all other nhntn½ in
phloxine. Scale bar: 10 pm.	Æ	v n
shaded sandstone bluffs, on corticolous Punctelia graminicola,
16 April 2005, R. C. Harris 50901 (NY - holotype).
MycoBank: MB844631
Basidiomata inducing galls on the host thallus; galls initially
simple, roundish, with a constricted base, often irregular in
form, 0.3-0.4 mm diam., concolorous to the thallus, soon
medium to dark brown in delimited areas, later becoming
lobiform, often with a concave to foveolate upper surface
and up to 1 mm diam. Context hyphae not observed; sub-
basidial hyphae thick-walled, 2-3 pm diam.; haustoria un-
known. Hymenium containing numerous probasidia and
some subbasidial hyphae, mixed with the host cortical cells;
probasidial initials shortly ellipsoid, thick-walled, basal
clamp not observed. Basidia, when mature, composed of
an ellipsoid, thick-walled probasidium, 9-17 x 5.5-11 pm,
and a cylindrical, generally bent, thin-walled, often decidu-
ous meiosporangium (upper part), 25-40 x 4-5 pm; upper
part with 2-3 transverse septa, individual cells collapsing
after spore production; epibasidia growing upwards, subu-
late, 1-2 pm thick, 4-5 pm long. Basidiospores ellipsoid to
fusiform, more or less symmetrical, with a distinct apiculus
at the lower end, 5-9 x 4-6.5 pm (apiculus not included).
Cyphobasidium puncteliicola
Asexual stage unknown. Yeast cells abundant in some par
of the hymenium, subspherical to shortly ellipsoid, 2-2.5
1.7-2.3 pm, budding and germination not observed.
Notes. This species induces the formation of conspicuoi;
galls on the host thallus, resembling somewhat those of Cj
phobasidium hypogymniicola, which become, howeve
much larger, up to 17 mm diam., and bullate. Microscopically
C. hypogymniicola has larger basidiospores, 7-12 x 4-7
Ecology and host. On the corticolous thallus of Punclelit
graminicola.
Distribution. North America (USA: Missouri), known onl
from the type locality.
Cyphobasidium ramalinicola P. Pinault & Diederich,
sp. nov.
Diagnosis: Characterized by the resupinate, poorly delimited
pale orange-brown basidiomata, 1-15 mm diam., on the thal
lus of Ramalina fraxinea, ellipsoid probasidia, 10ù14(ù18) i
7-9.5 pm, cylindrical/ 3-transseptate meiosporangia, 35-5!
x 4-6.5 pm, subulate epibasidia, 1.5-2 pm thick and 3-5 pn
long, and ellipsoid basidiospores, 6-8.5 x 4.5-6 pm.
Etymology: From Ramalina, die host lichen, and incola, dweller.
Type: France, Puy-de-D(me, commune de Saint-GenFs-Cham
panelle, Chatrat, 45.7301¦N, 3.0132¦E, 910 m, on Fraxinus, or
Ramalina fraxinea, 9 April 2022, P. Pinault 77 (BR - holotype)
MycoBank: MB844632
Basidiomata resupinate over thin or thick, often irregular
frequently poor delimited gall-like swellings of the hosi
thallus, waxy-gelatinous, pale orange-brown, 1-15 mrr
diam. Context hyphae not observed; subbasidial hyphae